Hotspot

rpl32-trnL-UAG

SSC · 115814-116602 · 789 bp

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Pi 0.028

MarkerSeek score 60.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 789
variable_sites: 22
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TAGACACGGCACTATGAGCT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1253-1270 bp	1.0	80.7
rpl32-trnL-UAG_p2	TAGACACGGCACTATGAGCT	ACTGAGATTGTAGGGCAGGT	50.0	50.0	58.2	58.0	1775-1792 bp	1.0	80.5
rpl32-trnL-UAG_p3	TAGACACGGCACTATGAGCT	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.2	57.9	1712-1729 bp	1.0	80.4
rpl32-trnL-UAG_p4	TAGACACGGCACTATGAGCT	GGGTAAAAATGGCACTTGGC	50.0	50.0	58.2	57.9	1700-1717 bp	1.0	80.2
rpl32-trnL-UAG_p5	TAGACACGGCACTATGAGCT	ACTGAAAATAACGACCCGCA	50.0	45.0	58.2	57.8	1845-1862 bp	1.0	79.4