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rpl33-rps18

LSC · 69732-69913 · 182 bp

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Pi 0.0301
MarkerSeek score 65.80
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
182
variable_sites
5
pi
0.0301
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TGTCGCTATTGTCACAAGCA CAATTCGATCCCCCGATTCA 45.0 50.0 57.8 57.7 299-315 bp 1.0 82.0
rpl33-rps18_p2 TGTCGCTATTGTCACAAGCA TCGATCCCCCGATTCAATTG 45.0 50.0 57.8 57.7 295-311 bp 1.0 82.0
rpl33-rps18_p3 TGTCGCTATTGTCACAAGCA TCAATTGGGGGAAAACGCTT 45.0 45.0 57.8 58.3 282-298 bp 1.0 81.8
rpl33-rps18_p4 TGTCGCTATTGTCACAAGCA TTCAATTGGGGGAAAACGCT 45.0 45.0 57.8 58.3 283-299 bp 1.0 81.8
rpl33-rps18_p5 TCGACACAATACACCCAGTG TCGATCCCCCGATTCAATTG 50.0 50.0 57.6 57.7 335-351 bp 1.0 79.3