Hotspot

atpB-rbcL

LSC · 56252-57094 · 843 bp

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Pi 0.0143

MarkerSeek score 67.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 12
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.9	56.6	951-1012 bp	1.0	69.1
atpB-rbcL_p2	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.5	56.6	952-1013 bp	1.0	66.2
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	45.0	42.9	57.9	57.2	951-1012 bp	1.0	65.3
atpB-rbcL_p4	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	975-1036 bp	1.0	64.8
atpB-rbcL_p5	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	914-975 bp	1.0	64.7