Hotspot

ndhC-trnV-UAC

LSC · 51723-53292 · 1570 bp

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Pi 0.0157
MarkerSeek score 55.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1570
variable_sites
19
pi
0.0157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.77
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1346-1701 bp 1.0 82.3
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1345-1700 bp 1.0 79.6
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1334-1689 bp 1.0 78.4
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT AAGGTCTACGGTTCGAGTCC 50.0 55.0 57.9 58.8 1344-1699 bp 1.0 77.3
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT GAGAAGGTCTACGGTTCGAG 50.0 55.0 57.9 57.2 1347-1702 bp 1.0 77.3