Hotspot

psbC-trnS-UGA

LSC · 36299-36425 · 127 bp

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Pi 0.063

MarkerSeek score 71.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 8
pi: 0.063
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	180-187 bp	1.0	80.5
psbC-trnS-UGA_p2	TTCCATGACCCCTCTTAGCT	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.7	58.2	172-179 bp	1.0	79.7
psbC-trnS-UGA_p3	TTGTGGGTCATTTGTGGCAT	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.3	58.2	260-267 bp	1.0	78.4
psbC-trnS-UGA_p4	GCAGCAGGATTTGAAAAGGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.6	58.2	219-226 bp	1.0	78.1
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.4	58.2	223-230 bp	1.0	78.1