Hotspot

rps16-trnQ-UUG

LSC · 5772-7146 · 1375 bp

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Pi 0.0284

MarkerSeek score 64.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1375
variable_sites: 33
pi: 0.0284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1262-1448 bp	1.0	84.2
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1273-1459 bp	1.0	83.7
rps16-trnQ-UUG_p3	CCACAGCGAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	1283-1469 bp	1.0	83.3
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1266-1452 bp	1.0	79.5
rps16-trnQ-UUG_p5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.2	58.1	1274-1460 bp	1.0	79.2