Hotspot

trnK-UUU-rps16

LSC · 4041-4625 · 585 bp

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Pi 0.0363
MarkerSeek score 70.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
585
variable_sites
21
pi
0.0363
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCAGAATTATTTAAAGAAAGAGCTTCG 50.0 33.3 58.2 58.0 644-649 bp 1.0 43.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCAGAATTATTTAAAGAAAGAGCTTCG 50.0 33.3 58.2 58.0 643-648 bp 1.0 43.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT ACTGTTCATGATATTTTAAGGAAAGCA 50.0 29.6 58.2 58.0 668-673 bp 1.0 43.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT ACTGTTCATGATATTTTAAGGAAAGCA 50.0 29.6 58.2 58.0 667-672 bp 1.0 43.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGTTCATGATATTTTAAGGAAAGCAGA 50.0 29.6 58.2 57.8 665-670 bp 1.0 43.2