Hotspot

psbA-trnK-UUU

LSC · 1145-1383 · 239 bp

Back to Leucojum

Pi 0.0377

MarkerSeek score 64.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 9
pi: 0.0377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GCTTGTACTTTCGCGTCTCT	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	58.3	57.9	365 bp	1.0	78.8
psbA-trnK-UUU_p2	CCCCACAGGCTTGTACTTTC	GTTTGGGGAGGGGTCTTTTT	55.0	50.0	58.5	57.9	373 bp	1.0	77.4
psbA-trnK-UUU_p3	GCTTGTACTTTCGCGTCTCT	TTTGGGGAGGGGTCTTTTTC	50.0	50.0	58.3	57.6	364 bp	1.0	76.4
psbA-trnK-UUU_p4	GCTTGTACTTTCGCGTCTCT	AAAATGCAAGCACGGTTTGG	50.0	45.0	58.3	58.4	379 bp	1.0	76.1
psbA-trnK-UUU_p5	ATCCAGTTACAGAAGCGACC	GTTTGGGGAGGGGTCTTTTT	50.0	50.0	57.3	57.9	391 bp	1.0	75.7