Hotspot

psbH

LSC · 75334-75555 · 222 bp

Back to Leucocoryne

Pi 0.0045

MarkerSeek score 42.60

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 222
variable_sites: 1
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	ACAAAGTCAACAGATCTCAACCA	TGAAAAACTAGGGCTTCATAGT	39.1	36.4	58.2	55.1	294 bp	1.0	40.3
psbH_p2	AGTCAACAGATCTCAACCAATGA	TGAAAAACTAGGGCTTCATAGT	39.1	36.4	57.8	55.1	290 bp	1.0	40.3
psbH_p3	GATCACAACGGATCTACGAT	TGAAAAACTAGGGCTTCATAGT	45.0	36.4	54.8	55.1	349 bp	1.0	40.2
psbH_p4	GATCACAACGGATCTACGAT	AACTAGGGCTTCATAGTTCT	45.0	40.0	54.8	53.0	344 bp	1.0	40.2
psbH_p5	GATCACAACGGATCTACGAT	ACTAGGGCTTCATAGTTCTT	45.0	40.0	54.8	53.0	343 bp	1.0	40.2