Hotspot

psbD

LSC · 32967-34028 · 1062 bp

Back to Leucocoryne

Pi 0.00282

MarkerSeek score 60.40

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	ACGTGTTCGGAAATGGTTGA	TACGTGAGCCCCAAGTAGTT	45.0	50.0	58.0	58.4	1214 bp	1.0	76.9
psbD_p2	TGACGTGTTCGGAAATGGTT	TACGTGAGCCCCAAGTAGTT	45.0	50.0	58.0	58.4	1216 bp	1.0	76.9
psbD_p3	ATGACGTGTTCGGAAATGGT	TACGTGAGCCCCAAGTAGTT	45.0	50.0	57.8	58.4	1217 bp	1.0	75.9
psbD_p4	AAGGGGCATTGAACGAAGAA	TACGTGAGCCCCAAGTAGTT	45.0	50.0	57.7	58.4	1279 bp	1.0	75.0
psbD_p5	GCATGCCCTGGAAATGAGAT	TACGTGAGCCCCAAGTAGTT	50.0	50.0	58.3	58.4	1235 bp	1.0	74.9