Hotspot

ndhG

SSC · 119438-119968 · 531 bp

Back to Leucocoryne

Pi 0.00377

MarkerSeek score 50.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACTTCCCATTTTCCCTTTTTCA	GAACGCCCTTCTCTTTCCTG	36.4	55.0	56.3	58.6	671 bp	1.0	46.4
ndhG_p2	AACTTCCCATTTTCCCTTTTTCA	GAACGCCCTTCTCTTTCCTG	34.8	55.0	57.0	58.6	672 bp	1.0	44.1
ndhG_p3	ACTTCCCATTTTCCCTTTTTCA	GCCCTTCTCTTTCCTGGACT	36.4	55.0	56.3	59.0	667 bp	1.0	42.8
ndhG_p4	ACTTCCCATTTTCCCTTTTTCAT	GAACGCCCTTCTCTTTCCTG	34.8	55.0	56.8	58.6	671 bp	1.0	42.4
ndhG_p5	ACTTCCCATTTTCCCTTTTTCA	GGAACGCCCTTCTCTTTCC	36.4	57.9	56.3	58.1	672 bp	1.0	41.7