Hotspot

petA-psbJ

LSC · 63743-64834 · 1092 bp

Back to Leucocoryne

Pi 0.00275

MarkerSeek score 60.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1092
variable_sites: 3
pi: 0.00275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTTT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.4	58.1	1276 bp	1.0	74.4
petA-psbJ_p2	AGGCCTTTTGTTTTTCTTGGC	GCTGGTATTCCTGTGATCGG	42.9	55.0	58.1	58.1	1270 bp	1.0	70.5
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTTT	TTCCTATTCCGGGTTGGGTT	45.0	50.0	57.4	58.6	1232 bp	1.0	70.4
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTTTT	GCTGGTATTCCTGTGATCGG	42.9	55.0	58.1	58.1	1276 bp	1.0	70.4
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTTT	TATTCCGGGTTGGGTTCATC	45.0	50.0	57.4	57.3	1228 bp	1.0	69.7