Hotspot

ndhC-trnV-UAC

LSC · 50533-52252 · 1720 bp

Back to Leucocoryne

Pi 0.00657

MarkerSeek score 64.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1720
variable_sites: 11
pi: 0.00657
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.9	57.9	1810-1840 bp	1.0	80.7
ndhC-trnV-UAC_p2	AATTGGGGCTAAAACTCCGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.2	57.9	1814-1844 bp	1.0	80.6
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAAGAAAATGC	50.0	45.0	57.9	57.9	1903-1933 bp	1.0	80.4
ndhC-trnV-UAC_p4	AATTGGGGCTAAAACTCCGG	AGCCCAATGCAAGAAAATGC	50.0	45.0	58.2	57.9	1907-1937 bp	1.0	80.3
ndhC-trnV-UAC_p5	GGTCCTTCACTAATTGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.2	57.9	1825-1855 bp	1.0	79.9