Hotspot

trnT-GGU-psbD

LSC · 31972-32966 · 995 bp

Back to Leucocoryne

Pi 0.00302

MarkerSeek score 53.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 3
pi: 0.00302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1120-1122 bp	1.0	76.1
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.3	57.7	1104-1106 bp	1.0	76.1
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.3	57.6	1119-1121 bp	1.0	74.9
trnT-GGU-psbD_p4	TGGTAGAGTAACGCCATGGT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.4	58.2	1142-1144 bp	1.0	74.9
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	CCCTCCGTAACCAATCATCC	50.0	55.0	58.4	57.7	1126-1128 bp	1.0	74.8