Hotspot

trnS-GCU-trnG-GCC

LSC · 8127-9199 · 1073 bp

Back to Leucocoryne

Pi 0.00466

MarkerSeek score 61.40

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 5
pi: 0.00466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	AATCTGCCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	1179-1201 bp	1.0	65.2
trnS-GCU-trnG-GCC_p2	CAATCTGCCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	1180-1202 bp	1.0	65.1
trnS-GCU-trnG-GCC_p3	CGGAGAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	1233-1255 bp	1.0	64.7
trnS-GCU-trnG-GCC_p4	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	1171-1193 bp	1.0	64.0
trnS-GCU-trnG-GCC_p5	AGTCTGAAGTAGAGGGAGCG	AACCGAAAGACCCCTTAACT	55.0	45.0	58.2	56.4	1251 bp	0.5	45.0