Hotspot

matK-trnK-UUU

LSC · 3248-4004 · 757 bp

Back to Leucocoryne

Pi 0.00265

MarkerSeek score 60.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 2
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GTGTTGTTGCCGGGATCTAT	TCTGGGTTGCTAACTCAACG	50.0	50.0	58.0	57.8	848-858 bp	1.0	78.7
matK-trnK-UUU_p2	GTGTTGTTGCCGGGATCTAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	832-842 bp	1.0	78.6
matK-trnK-UUU_p3	GTGTTGTTGCCGGGATCTAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	831-841 bp	1.0	78.6
matK-trnK-UUU_p4	ATAGGAAGTGTTGTTGCCGG	TCTGGGTTGCTAACTCAACG	50.0	50.0	58.2	57.8	855-865 bp	1.0	77.5
matK-trnK-UUU_p5	ATAGGAAGTGTTGTTGCCGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.2	58.2	838-848 bp	1.0	77.5