Hotspot

ycf1

SSC · 121183-126720 · 5538 bp

Pi 0.0164

MarkerSeek score 47.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 91
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCATGTCCTTGCTCTTCGTA	CGATGGGATGAGGCTTTGAA	50.0	50.0	58.0	58.0	302 bp	1.0	82.2
ycf1_p2	GCATGTCCTTGCTCTTCGTA	ACGAAAGCAGAACTCCGTTT	50.0	45.0	58.0	58.1	353 bp	1.0	82.0
ycf1_p3	TCATGTGAAACTTGGCTGCT	CGATGGGATGAGGCTTTGAA	45.0	50.0	58.0	58.0	521 bp	1.0	81.2
ycf1_p4	GCATGTCCTTGCTCTTCGTA	TCAATTGCGATGGGATGAGG	50.0	50.0	58.0	58.0	309 bp	0.5	59.7
ycf1_p5	TCATGTGAAACTTGGCTGCT	TCAATTGCGATGGGATGAGG	45.0	50.0	58.0	58.0	528 bp	0.5	59.7