Hotspot

ndhI-ndhA

SSC · 117039-117124 · 86 bp

Back to Leucas

Pi 0.0233
MarkerSeek score 65.90
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
86
variable_sites
2
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 GCTCGTATTGTTTGTTGGCC ACCGAGACTGAGAATGGACC 50.0 55.0 58.0 58.8 248 bp 1.0 74.0
ndhI-ndhA_p2 GCTCGTATTGTTTGTTGGCC CCGAGACTGAGAATGGACCA 50.0 55.0 58.0 58.8 247 bp 1.0 74.0
ndhI-ndhA_p3 TGACCAATGTATCTGGCAGC ACCGAGACTGAGAATGGACC 50.0 55.0 58.0 58.8 266 bp 1.0 73.8
ndhI-ndhA_p4 TGACCAATGTATCTGGCAGC CCGAGACTGAGAATGGACCA 50.0 55.0 58.0 58.8 265 bp 1.0 73.8
ndhI-ndhA_p5 CAATGTATCTGGCAGCTCGT ACCGAGACTGAGAATGGACC 50.0 55.0 58.1 58.8 262 bp 1.0 73.7