Hotspot

rpl20-rps12

LSC · 67659-68396 · 738 bp

Back to Leucas

Pi 0.0136
MarkerSeek score 63.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
738
variable_sites
10
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TGAATGAGACCCCCGAAAAC CGCCGAGGAACATGTACTAG 50.0 55.0 57.8 57.9 852-867 bp 1.0 78.4
rpl20-rps12_p2 AGACCCCCGAAAACTTGATG CGCCGAGGAACATGTACTAG 50.0 55.0 57.8 57.9 846-861 bp 1.0 78.4
rpl20-rps12_p3 ATGAGACCCCCGAAAACTTG CGCCGAGGAACATGTACTAG 50.0 55.0 57.8 57.9 849-864 bp 1.0 78.4
rpl20-rps12_p4 TGAATGAGACCCCCGAAAAC ACAGCCCATTCGAAATGTCA 50.0 45.0 57.8 57.8 904-919 bp 1.0 77.7
rpl20-rps12_p5 AGACCCCCGAAAACTTGATG ACAGCCCATTCGAAATGTCA 50.0 45.0 57.8 57.8 898-913 bp 1.0 77.7