Hotspot

psbE-petL

LSC · 63801-64727 · 927 bp

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Pi 0.00647
MarkerSeek score 62.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
927
variable_sites
6
pi
0.00647
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1119-1126 bp 1.0 72.5
psbE-petL_p2 ACTGACGAATAACCAACCCG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1132-1139 bp 1.0 72.2
psbE-petL_p3 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1042-1049 bp 1.0 71.7
psbE-petL_p4 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCA 45.0 50.0 58.2 57.1 1128-1135 bp 1.0 71.5
psbE-petL_p5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1127-1134 bp 1.0 71.4