Hotspot

atpB-rbcL

LSC · 53470-54264 · 795 bp

Back to Leucas

Pi 0.0164
MarkerSeek score 55.00
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
795
variable_sites
13
pi
0.0164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AGAAGTAGTAGGATTCATTATCAT GCTTTGAATCCAACACCTGC 29.2 50.0 52.5 57.9 863-887 bp 1.0 41.2
atpB-rbcL_p2 AGAAGTAGTAGGATTCATTATCAT AACACCCGCTTTGAATCCAA 29.2 45.0 52.5 58.3 870-894 bp 1.0 41.2
atpB-rbcL_p3 AGAAGTAGTAGGATTCATTATCAT CGCTTTGAATCCAACACCTG 29.2 50.0 52.5 57.7 864-888 bp 1.0 41.2
atpB-rbcL_p4 AGAAGTAGTAGGATTCATTATCAT TAACACCCGCTTTGAATCCA 29.2 45.0 52.5 57.4 871-895 bp 1.0 41.2
atpB-rbcL_p5 AGAAGTAGTAGGATTCATTATCAT CCGCTTTGAATCCAACACCT 29.2 50.0 52.5 58.8 865-889 bp 1.0 41.2