Hotspot

ndhC-trnI-AAU

LSC · 49499-50464 · 966 bp

Back to Leucas

Pi 0.0187
MarkerSeek score 58.10
Primer available yes
Label ndhC-trnI-AAU

Feature Metadata

label_name
ndhC-trnI-AAU
feature_type
igs
region
LSC
length_bp
966
variable_sites
18
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnI-AAU_p1 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1097-1102 bp 1.0 79.4
ndhC-trnI-AAU_p2 AATTGGGGCTAAAATCCCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1101-1106 bp 1.0 78.3
ndhC-trnI-AAU_p3 GGGGCTAAAATCCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1096-1101 bp 1.0 76.6
ndhC-trnI-AAU_p4 AATTGGGGCTAAAATCCCGG GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.2 57.5 1100-1105 bp 1.0 75.6
ndhC-trnI-AAU_p5 GGGGCTAAAATCCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1085-1090 bp 1.0 75.5