Hotspot

trnT-UGU-trnL-UAA

LSC · 45577-46230 · 654 bp

Back to Leucas

Pi 0.0138
MarkerSeek score 66.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
654
variable_sites
9
pi
0.0138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG GGTTTCCCACACTAAGGCTC 55.0 55.0 57.9 58.2 754-775 bp 1.0 78.5
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 715-736 bp 1.0 77.9
trnT-UGU-trnL-UAA_p3 TAGGAAAAAGCCGGCTATCG GGTTTCCCACACTAAGGCTC 50.0 55.0 57.8 58.2 805-826 bp 1.0 77.6
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT GGTTTCCCACACTAAGGCTC 50.0 55.0 57.8 58.2 786-807 bp 1.0 77.6
trnT-UGU-trnL-UAA_p5 TAGGAAAAAGCCGGCTATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 766-787 bp 1.0 77.1