Hotspot

atpH-atpI

LSC · 13705-14693 · 989 bp

Back to Leucas

Pi 0.0198
MarkerSeek score 58.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
989
variable_sites
19
pi
0.0198
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 CTTGGACTACGAACGGGAAG TGCTTTGGCTTTACTCACGT 55.0 45.0 58.0 58.0 1729-1758 bp 1.0 82.2
atpH-atpI_p2 CTCATCTGCGAATCAGCCTT TGCTTTGGCTTTACTCACGT 50.0 45.0 58.0 58.0 1682-1711 bp 1.0 81.9
atpH-atpI_p3 CTTGGACTACGAACGGGAAG GCCGCACCTACGAATGATAT 55.0 50.0 58.0 57.9 1761-1790 bp 1.0 81.8
atpH-atpI_p4 CTTGGACTACGAACGGGAAG GGCAAATAGGGGGTTTCCAA 55.0 50.0 58.0 58.1 2060-2089 bp 1.0 81.7
atpH-atpI_p5 CTTGGACTACGAACGGGAAG TTGGCAAATAGGGGGTTTCC 55.0 50.0 58.0 58.1 2062-2091 bp 1.0 81.7