Hotspot

ycf4

LSC · 58964-59518 · 555 bp

Back to Leucanthemum

Pi 0.0562

MarkerSeek score 78.40

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 62
pi: 0.0562
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	GCAATCGCAATGGCTTCTTT	GGAATTCCTGAGCCCAAGAG	45.0	55.0	58.0	58.0	1643-1644 bp	0.75	69.9
ycf4_p2	GCAATCGCAATGGCTTCTTT	TGAGAGCGAAATCCACCAAG	45.0	50.0	58.0	57.9	1297-1298 bp	0.75	69.6
ycf4_p3	GCAATCGCAATGGCTTCTTT	TGCTTTGACTCGAAGGGATG	45.0	50.0	58.0	57.9	1084-1085 bp	0.75	69.6
ycf4_p4	GCAATCGCAATGGCTTCTTT	GCTTTGACTCGAAGGGATGT	45.0	50.0	58.0	57.9	1083-1084 bp	0.75	69.6
ycf4_p5	ATAGACCCAAATTGGCGGAC	GGAATTCCTGAGCCCAAGAG	50.0	55.0	57.9	58.0	1473-1474 bp	0.75	69.5