Hotspot

rps15

SSC · 120485-120763 · 279 bp

Back to Leucanthemum

Pi 0.327

MarkerSeek score 63.20

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 137
pi: 0.327
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	794-819 bp	0.5	63.1
rps15_p2	TGAGGTAAATCAGCAGCGAC	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.0	58.0	1225-1250 bp	0.5	62.2
rps15_p3	TGTTCTGGCGCATTTACAGT	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	982-1007 bp	0.5	62.0
rps15_p4	TGAGGTAAATCAGCAGCGAC	GTCCTGTCTTACATCCCCCT	50.0	55.0	58.0	58.1	1164 bp	0.25	49.0
rps15_p5	AACACCATGCATTGATGGGT	GTCCTGTCTTACATCCCCCT	45.0	55.0	58.1	58.1	733 bp	0.25	48.6