Hotspot

ndhI

SSC · 116432-116932 · 501 bp

Back to Leucanthemum

Pi 0.343

MarkerSeek score 62.30

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 258
pi: 0.343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGACCACGGAAGTTACTCCA	TTTGTGACCTTTGAGGAGCC	50.0	50.0	58.0	58.0	1880 bp	0.5	60.1
ndhI_p2	AGACCACGGAAGTTACTCCA	GTACGGTTCTGCAATAGGGG	50.0	55.0	58.0	58.1	1840 bp	0.5	59.8
ndhI_p3	AGACCACGGAAGTTACTCCA	AATAGGGGTGGGAACAGTGA	50.0	50.0	58.0	57.9	1828 bp	0.5	59.8
ndhI_p4	AGACCACGGAAGTTACTCCA	GACCTTTGAGGAGCCGTATG	50.0	55.0	58.0	58.1	1875 bp	0.5	59.7
ndhI_p5	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1369 bp	0.5	59.7