Hotspot

ndhD

SSC · 112945-114447 · 1503 bp

Back to Leucanthemum

Pi 0.275

MarkerSeek score 71.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 620
pi: 0.275
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAGTGATTCACGCGGTTCT	CATGTGTGCCACGTAAGGTA	50.0	50.0	58.0	57.9	2169 bp	0.5	59.3
ndhD_p2	GTAGTGATTCACGCGGTTCT	TGTGTGCCACGTAAGGTATG	50.0	50.0	58.0	57.9	2167 bp	0.5	59.3
ndhD_p3	GTAGTGATTCACGCGGTTCT	ACAGAGGATTGTGTTGGCTG	50.0	50.0	58.0	58.1	1855 bp	0.5	59.3
ndhD_p4	GTAGTGATTCACGCGGTTCT	CAGAGGATTGTGTTGGCTGT	50.0	50.0	58.0	58.1	1854 bp	0.5	59.3
ndhD_p5	GTAGTGATTCACGCGGTTCT	CAGAAACCTCTCCTTGAGCC	50.0	55.0	58.0	57.9	1734 bp	0.5	59.2