Hotspot

ndhA

SSC · 117009-119210 · 2202 bp

Back to Leucanthemum

Pi 0.28

MarkerSeek score 79.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2202
variable_sites: 922
pi: 0.28
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCAATATACCTTGCGGC	AGAGTTGAAGCACCAAAGGG	50.0	50.0	58.0	58.0	2548-2552 bp	0.5	59.8
ndhA_p2	TGACCAATATACCTTGCGGC	AGTTGAAGCACCAAAGGGAG	50.0	50.0	58.0	58.0	2546-2550 bp	0.5	59.8
ndhA_p3	TGACCAATATACCTTGCGGC	GAGTTGAAGCACCAAAGGGA	50.0	50.0	58.0	58.0	2547-2551 bp	0.5	59.7
ndhA_p4	TGACCAATATACCTTGCGGC	GTTGAAGCACCAAAGGGAGA	50.0	50.0	58.0	58.0	2545-2549 bp	0.5	59.7
ndhA_p5	TGACCAATATACCTTGCGGC	CAACAGGCGCTAGAAGGAAT	50.0	50.0	58.0	58.0	2707-2711 bp	0.5	59.6