Hotspot

rpl32-trnL-UAG

SSC · 110634-111587 · 954 bp

Back to Leucanthemum

Pi 0.266

MarkerSeek score 81.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 954
variable_sites: 382
pi: 0.266
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCGGCATTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1071-1104 bp	0.5	61.7
rpl32-trnL-UAG_p2	GGCGGCATTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1072-1105 bp	0.5	61.6
rpl32-trnL-UAG_p3	AAAGGATATTGGGCGGCATT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1082-1115 bp	0.5	61.6
rpl32-trnL-UAG_p4	AAAGGATATTGGGCGGCATT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1083-1116 bp	0.5	61.6
rpl32-trnL-UAG_p5	GGCGGCATTAAAAGCTTTGT	CCCGAATCAATCCAACGAGT	45.0	50.0	57.9	58.0	1419-1452 bp	0.5	61.0