Hotspot

trnW-CCA-trnP-UGG

LSC · 64967-65141 · 175 bp

Back to Leucanthemum

Pi 0.131

MarkerSeek score 80.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 45
pi: 0.131
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	257-263 bp	1.0	89.1
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	258-264 bp	1.0	89.1
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	246-252 bp	1.0	89.1
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	244-250 bp	1.0	89.1
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	247-253 bp	1.0	89.1