Hotspot

petA-psbJ

LSC · 61668-62367 · 700 bp

Back to Leucanthemum

Pi 0.135

MarkerSeek score 83.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 185
pi: 0.135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTAGGCGGAAACAGAGGAAG	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1818-2245 bp	1.0	88.7
petA-psbJ_p2	CTGACGGGAGCAAGAGTAAC	TTAGGAGGCCTCAATGACCA	55.0	50.0	58.0	58.0	1620-1686 bp	0.75	70.2
petA-psbJ_p3	GTAGGCGGAAACAGAGGAAG	TTAGGAGGCCTCAATGACCA	55.0	50.0	58.0	58.0	1654-1720 bp	0.75	70.2
petA-psbJ_p4	CTGACGGGAGCAAGAGTAAC	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1784-1850 bp	0.75	70.1
petA-psbJ_p5	CTGACGGGAGCAAGAGTAAC	TGGAGGGAAGTGGGGTAAAT	55.0	50.0	58.0	57.9	1218-1284 bp	0.75	70.1