Hotspot

petA-psbJ

LSC · 72352-73685 · 1334 bp

Back to Leucaena

Pi 0.0156

MarkerSeek score 71.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1334
variable_sites: 20
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCTCTTGTTCTTCGTGGCA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1447-1461 bp	1.0	79.8
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1457-1471 bp	1.0	78.9
petA-psbJ_p3	TGTTCTTCGTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1441-1455 bp	1.0	78.9
petA-psbJ_p4	TTGTTCTTCGTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1442-1456 bp	1.0	78.9
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1462-1476 bp	1.0	78.4