Hotspot

rbcL-accD

LSC · 65756-66846 · 1091 bp

Back to Leucaena

Pi 0.0294

MarkerSeek score 64.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 31
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TTTGGTCGTCACTTCCACTG	50.0	50.0	58.0	58.1	1263-1351 bp	1.0	83.4
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TTTGGTCGTCACTTCCACTG	55.0	50.0	58.0	58.1	1278-1366 bp	1.0	83.3
rbcL-accD_p3	CGCTGGTACCGTAGTAGGTA	TTTGGTCGTCACTTCCACTG	55.0	50.0	58.0	58.1	1636-1724 bp	1.0	82.5
rbcL-accD_p4	GAGCTGTATTTGCCCGAGAA	TTTGGTCGTCACTTCCACTG	50.0	50.0	58.0	58.1	1859-1947 bp	1.0	82.3
rbcL-accD_p5	AAGAGCTGTATTTGCCCGAG	TTTGGTCGTCACTTCCACTG	50.0	50.0	58.0	58.1	1861-1949 bp	1.0	82.3