Hotspot

trnR-UCU-atpA

LSC · 12981-13485 · 505 bp

Back to Leucaena

Pi 0.0487

MarkerSeek score 75.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 22
pi: 0.0487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	ACTTCAGGAACAAGTAGAAAA	47.6	33.3	57.3	52.6	555-565 bp	1.0	47.1
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	ACTTCAGGAACAAGTAGAAAAA	47.6	31.8	57.3	53.5	555-565 bp	1.0	47.1
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	ACTTCAGGAACAAGTAGAAAA	42.9	33.3	56.6	52.6	554-564 bp	1.0	47.1
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	ACTTCAGGAACAAGTAGAAAA	47.6	33.3	56.9	52.6	590-600 bp	1.0	47.0
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	ACTTCAGGAACAAGTAGAAAAA	47.6	31.8	56.9	53.5	590-600 bp	1.0	47.0