Hotspot

trnS-GCU-trnG-UCC

LSC · 10036-11623 · 1588 bp

Back to Leucaena

Pi 0.0273
MarkerSeek score 61.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1588
variable_sites
31
pi
0.0273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1238-1668 bp 1.0 77.5
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1237-1667 bp 1.0 77.4
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA GGATCCATAGAACGAATCGCA 50.0 47.6 58.2 58.0 1292-1722 bp 1.0 76.2
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 1227-1657 bp 1.0 75.8
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 1226-1656 bp 1.0 75.8