Hotspot

trnQ-UUG-psbK

LSC · 8036-8870 · 835 bp

Back to Leucaena

Pi 0.0414

MarkerSeek score 68.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 27
pi: 0.0414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	AAAACCCGTTGCCTTACCG	ACAGACCAAACTAAAGATATTAAGCA	52.6	30.8	58.7	56.9	755-896 bp	1.0	46.6
trnQ-UUG-psbK_p2	CAAAACCCGTTGCCTTACC	ACAGACCAAACTAAAGATATTAAGCA	52.6	30.8	57.1	56.9	756-897 bp	1.0	46.6
trnQ-UUG-psbK_p3	CAAAACCCGTTGCCTTACCG	ACAGACCAAACTAAAGATATTAAGCA	55.0	30.8	60.0	56.9	756-897 bp	1.0	46.6
trnQ-UUG-psbK_p4	AAACCCGTTGCCTTACCG	ACAGACCAAACTAAAGATATTAAGCA	55.6	30.8	57.9	56.9	754-895 bp	1.0	46.6
trnQ-UUG-psbK_p5	AAAACCCGTTGCCTTACCG	CAGACCAAACTAAAGATATTAAGCA	52.6	32.0	58.7	55.4	754-895 bp	1.0	46.6