Hotspot

trnK-UUU-rps16

LSC · 4467-5848 · 1382 bp

Back to Leucaena

Pi 0.0244

MarkerSeek score 68.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1382
variable_sites: 32
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.6	58.0	1540-1634 bp	1.0	80.4
trnK-UUU-rps16_p2	GAGTCGCACTTAAAAGCCGA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.6	58.0	1541-1635 bp	1.0	80.3
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	45.0	45.0	57.3	58.0	1538-1632 bp	1.0	79.1
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	58.6	58.2	1545-1639 bp	1.0	78.7
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.6	58.2	1544-1638 bp	1.0	78.7