Hotspot

petF

SSC · 107625-107918 · 294 bp

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Pi 0.0159

MarkerSeek score 49.10

Primer available yes

Label petF

Feature Metadata

label_name: petF
feature_type: gene
region: SSC
length_bp: 294
variable_sites: 9
pi: 0.0159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF_p1	TGACGCCACATTGGGTTTAA	GGTGAAGCAGGGGAAGAAAA	45.0	50.0	58.0	58.0	1030-1054 bp	1.0	82.0
petF_p2	TTGACGCCACATTGGGTTTA	GGTGAAGCAGGGGAAGAAAA	45.0	50.0	58.0	58.0	1031-1055 bp	1.0	82.0
petF_p3	TGCCAAACAGCAGCTAAGAA	GGTGAAGCAGGGGAAGAAAA	45.0	50.0	58.0	58.0	1201-1225 bp	1.0	81.9
petF_p4	TGACGCCACATTGGGTTTAA	TCCTGGAGTAAAGGGACGTT	45.0	50.0	58.0	58.0	1338-1362 bp	1.0	81.7
petF_p5	TTGACGCCACATTGGGTTTA	TCCTGGAGTAAAGGGACGTT	45.0	50.0	58.0	58.0	1339-1363 bp	1.0	81.7