Hotspot

ilvH

LSC · 47657-48253 · 597 bp

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Pi 0.0148

MarkerSeek score 53.90

Primer available yes

Label ilvH

Feature Metadata

label_name: ilvH
feature_type: gene
region: LSC
length_bp: 597
variable_sites: 17
pi: 0.0148
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ilvH_p1	ACCCGTCAGCTTTTTCTGTT	TGCAAATCGTGAGGGTTCAA	45.0	45.0	57.9	58.0	1321-1327 bp	1.0	82.2
ilvH_p2	AAGCGAGGGAATGTTGCTAG	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.0	58.0	1613-1619 bp	1.0	82.0
ilvH_p3	AGACCCGTCAGCTTTTTCTG	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.1	58.0	1323-1329 bp	1.0	81.7
ilvH_p4	GGAATGTTGCTAGGAACCGT	TGCAAATCGTGAGGGTTCAA	50.0	45.0	57.9	58.0	1606-1612 bp	1.0	81.4
ilvH_p5	AAGCGAGGGAATGTTGCTAG	CGTTGATGCAAATCGTGAGG	50.0	50.0	58.0	57.8	1619-1625 bp	1.0	80.9