Hotspot

cp74

LSC · 62894-64471 · 1578 bp

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Pi 0.0113

MarkerSeek score 53.60

Primer available yes

Label cp74

Feature Metadata

label_name: cp74
feature_type: gene
region: LSC
length_bp: 1578
variable_sites: 35
pi: 0.0113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cp74_p1	TGGCAAGAATTCACTACGGG	TCTTATCACCTGACCCTGCA	50.0	50.0	57.9	58.0	2555-2570 bp	1.0	81.6
cp74_p2	GGCAAGAATTCACTACGGGT	TCTTATCACCTGACCCTGCA	50.0	50.0	57.9	58.0	2554-2569 bp	1.0	81.6
cp74_p3	TGGGCTGGTCGTACTTATCT	TCTTATCACCTGACCCTGCA	50.0	50.0	57.8	58.0	2681-2696 bp	1.0	81.2
cp74_p4	TTTGGCCAGTATCAGCTTGG	TCTTATCACCTGACCCTGCA	50.0	50.0	58.2	58.0	2572-2587 bp	1.0	81.2
cp74_p5	TGGCAAGAATTCACTACGGG	TATGTTGGGGCAAACAGAGG	50.0	50.0	57.9	57.8	2499-2514 bp	1.0	80.3