Hotspot

ftsH-psbH

SSC · 105199-105288 · 90 bp

Back to Lessonia

Pi 0.0389

MarkerSeek score 66.10

Primer available yes

Label ftsH-psbH

Feature Metadata

label_name: ftsH-psbH
feature_type: igs
region: SSC
length_bp: 90
variable_sites: 7
pi: 0.0389
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH-psbH_p1	AACGCGAGGCCAATTATCTT	CAAAAATTTTGACCCCGGCC	45.0	50.0	58.0	58.5	932 bp	0.75	66.8
ftsH-psbH_p2	AACGCGAGGCCAATTATCTT	TCAAAAATTTTGACCCCGGC	45.0	45.0	58.0	57.5	933 bp	0.75	66.4
ftsH-psbH_p3	AGCAACCTCTTTTAACGCGA	CAAAAATTTTGACCCCGGCC	45.0	50.0	58.1	58.5	945 bp	0.75	66.1
ftsH-psbH_p4	CGCGAGGCCAATTATCTTCT	CAAAAATTTTGACCCCGGCC	50.0	50.0	58.1	58.5	930 bp	0.75	66.1
ftsH-psbH_p5	AACCTTAATTCCTCGTGGGC	CAAAAATTTTGACCCCGGCC	50.0	50.0	57.9	58.5	998 bp	0.75	66.0