Hotspot

psbA-cbbx

LSC · 74618-75251 · 634 bp

Back to Lessonia

Pi 0.0203

MarkerSeek score 61.30

Primer available yes

Label psbA-cbbx

Feature Metadata

label_name: psbA-cbbx
feature_type: igs
region: LSC
length_bp: 634
variable_sites: 24
pi: 0.0203
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-cbbx_p1	CTGCTCCTTCTGTTGTTGGT	ACAGCTAGTAGTGTATTTAGTT	50.0	31.8	58.0	52.0	684-717 bp	1.0	43.8
psbA-cbbx_p2	CTGCTCCTTCTGTTGTTGGT	ACAGCTAGTAGTGTATTTAGTTT	50.0	30.4	58.0	52.9	684-717 bp	1.0	43.8
psbA-cbbx_p3	TGTTGCGATTTCTGCTCCTT	ACAGCTAGTAGTGTATTTAGTT	45.0	31.8	58.1	52.0	695-728 bp	1.0	43.7
psbA-cbbx_p4	TTCCTGTTGCGATTTCTGCT	ACAGCTAGTAGTGTATTTAGTT	45.0	31.8	58.1	52.0	699-732 bp	1.0	43.7
psbA-cbbx_p5	TGTTGCGATTTCTGCTCCTT	ACAGCTAGTAGTGTATTTAGTTT	45.0	30.4	58.1	52.9	695-728 bp	1.0	43.7