Hotspot

psbK-ccsA

LSC · 57791-58118 · 328 bp

Back to Lessonia

Pi 0.0315

MarkerSeek score 62.30

Primer available yes

Label psbK-ccsA

Feature Metadata

label_name: psbK-ccsA
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 20
pi: 0.0315
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-ccsA_p1	TCTGGTAACTTTGTTAGTAATATGA	AGGGCAAGGTTTACATAGCT	28.0	45.0	53.7	56.5	397-412 bp	1.0	44.0
psbK-ccsA_p2	TCTGGTAACTTTGTTAGTAATATGA	CGGGTTCTTTGTTGTTTGGA	28.0	45.0	53.7	56.8	442-457 bp	1.0	43.6
psbK-ccsA_p3	TCTGGTAACTTTGTTAGTAATATGA	TCGGGTTCTTTGTTGTTTGG	28.0	45.0	53.7	56.8	443-458 bp	1.0	43.6
psbK-ccsA_p4	TCTGGTAACTTTGTTAGTAATATGA	TCGGGTTCTTTGTTGTTTGGA	28.0	42.9	53.7	58.3	443-458 bp	1.0	43.6
psbK-ccsA_p5	TCTGGTAACTTTGTTAGTAATATGA	AGCAAGTATCGGGTTCTTTGT	28.0	42.9	53.7	57.6	451-466 bp	1.0	43.5