Hotspot

trnS-trnM

LSC · 38450-38456 · 7 bp

Back to Lessonia

Pi 0.167

MarkerSeek score 70.20

Primer available yes

Label trnS-trnM

Feature Metadata

label_name: trnS-trnM
feature_type: igs
region: LSC
length_bp: 7
variable_sites: 2
pi: 0.167
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnM_p1	TCGAACCCTCGGTACAAAGA	GACTCATAAGCCCTTGGTCG	50.0	55.0	58.4	58.1	126 bp	1.0	77.8
trnS-trnM_p2	TCGAACCCTCGGTACAAAGA	AGTAGGGGACTCATAAGCCC	50.0	55.0	58.4	57.9	133 bp	1.0	77.3
trnS-trnM_p3	TCGAACCCTCGGTACAAAGA	GTAGGGGACTCATAAGCCCT	50.0	55.0	58.4	57.9	132 bp	1.0	77.3
trnS-trnM_p4	CGAACCCTCGGTACAAAGAA	GACTCATAAGCCCTTGGTCG	50.0	55.0	57.6	58.1	125 bp	1.0	77.3
trnS-trnM_p5	TTCGAACCCTCGGTACAAAG	GACTCATAAGCCCTTGGTCG	50.0	55.0	57.6	58.1	127 bp	1.0	77.2