Hotspot

thiG-trnG

LSC · 35754-35886 · 133 bp

Back to Lessonia

Pi 0.0397

MarkerSeek score 69.20

Primer available yes

Label thiG-trnG

Feature Metadata

label_name: thiG-trnG
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 9
pi: 0.0397
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG-trnG_p1	CTGGGAGATCCAATTCCTGC	ATCGCTGGTTCTGTTAACCC	55.0	50.0	58.0	57.9	1259-1271 bp	1.0	81.5
thiG-trnG_p2	CAGTCCCAACAATAAGGCGA	ATCGCTGGTTCTGTTAACCC	50.0	50.0	57.9	57.9	724-736 bp	1.0	81.5
thiG-trnG_p3	CGGGTCAGGGAGAAGATAGT	ATCGCTGGTTCTGTTAACCC	55.0	50.0	57.9	57.9	1023-1035 bp	1.0	81.4
thiG-trnG_p4	TTGTCTACCTGCTTGCACAG	ATCGCTGGTTCTGTTAACCC	50.0	50.0	58.1	57.9	1383-1395 bp	1.0	80.7
thiG-trnG_p5	ATTCTCGGCCTAAAAACGCT	ATCGCTGGTTCTGTTAACCC	45.0	50.0	57.9	57.9	952 bp	0.25	48.2