Hotspot

trnY-cp30

LSC · 27468-27529 · 62 bp

Back to Lessonia

Pi 0.0618

MarkerSeek score 80.10

Primer available yes

Label trnY-cp30

Feature Metadata

label_name: trnY-cp30
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 7
pi: 0.0618
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-cp30_p1	AAGGGGGCGGATTGTAAATC	GTCGAGATGGTCCGGATTTT	50.0	50.0	57.9	57.7	196 bp	1.0	79.8
trnY-cp30_p2	AATCCGCTGGTTTTCCTACG	GTCGAGATGGTCCGGATTTT	50.0	50.0	58.3	57.7	180 bp	1.0	78.5
trnY-cp30_p3	TCATGGACTGTTAGGGTCGA	GTCGAGATGGTCCGGATTTT	50.0	50.0	57.8	57.7	230 bp	1.0	78.1
trnY-cp30_p4	GCTGGTTTTCCTACGTTGGT	GTCGAGATGGTCCGGATTTT	50.0	50.0	58.4	57.7	175 bp	1.0	77.5
trnY-cp30_p5	GTTAAAGGGGGCGGATTGTA	GTCGAGATGGTCCGGATTTT	50.0	50.0	57.6	57.7	200 bp	1.0	76.9