Hotspot

psaM-chlI

LSC · 6023-6136 · 114 bp

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Pi 0.0322

MarkerSeek score 65.40

Primer available yes

Label psaM-chlI

Feature Metadata

label_name: psaM-chlI
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 7
pi: 0.0322
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-chlI_p1	GGTTCCAAGCCTGTAAAGCT	CGTAATTCCCCTTCTTCCGG	50.0	55.0	58.1	58.1	1174-1188 bp	1.0	80.8
psaM-chlI_p2	GGTTCCAAGCCTGTAAAGCT	TTCGGCATGCATTCCAAAAC	50.0	45.0	58.1	57.9	1211-1225 bp	1.0	80.6
psaM-chlI_p3	GGTTCCAAGCCTGTAAAGCT	TTCTCGTTCGACTGTGTTCC	50.0	50.0	58.1	57.9	1103-1117 bp	1.0	80.3
psaM-chlI_p4	GGTTCCAAGCCTGTAAAGCT	CCTTCTCGTTCGACTGTGTT	50.0	50.0	58.1	57.9	1105-1119 bp	1.0	80.3
psaM-chlI_p5	GGTTCCAAGCCTGTAAAGCT	TGTGCTGGGTGTCGTATAGA	50.0	50.0	58.1	58.2	1129-1143 bp	1.0	80.1