Hotspot

ndhF-rpl32

SSC · 108432-109047 · 616 bp

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Pi 0.00694
MarkerSeek score 52.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
616
variable_sites
15
pi
0.00694
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAACTGGAAATGGAATAAAAGGT CGAAAAAGCTTTCAATCTTGTCC 37.5 39.1 57.4 57.2 671-813 bp 1.0 45.6
ndhF-rpl32_p2 AGGAACTGGAAATGGAATAAAAGG CGAAAAAGCTTTCAATCTTGTCC 37.5 39.1 57.1 57.2 672-814 bp 1.0 45.6
ndhF-rpl32_p3 GGAACTGGAAATGGAATAAAAGG CGAAAAAGCTTTCAATCTTGTCC 39.1 39.1 55.8 57.2 671-813 bp 1.0 45.6
ndhF-rpl32_p4 GGAACTGGAAATGGAATAAAAGGT AGCTTTCAATCTTGTCCAATATCC 37.5 37.5 57.4 57.3 665-807 bp 1.0 45.6
ndhF-rpl32_p5 GGAACTGGAAATGGAATAAAAGGT GCTTTCAATCTTGTCCAATATCCT 37.5 37.5 57.4 57.3 664-806 bp 1.0 45.6