Hotspot

rps3-rps19

LSC · 81618-82092 · 475 bp

Back to Lespedeza

Pi 0.00924

MarkerSeek score 51.20

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 16
pi: 0.00924
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CTGAAACCAAGCGGATTTAT	TAAACTTCCGAGGACATGCG	40.0	50.0	54.0	58.0	541-557 bp	1.0	54.0
rps3-rps19_p2	CTGAAACCAAGCGGATTTAT	TCCGAGGACATGCGAAAAAT	40.0	45.0	54.0	57.9	535-551 bp	1.0	53.2
rps3-rps19_p3	CTGAAACCAAGCGGATTTAT	TTCCGAGGACATGCGAAAAA	40.0	45.0	54.0	58.1	536-552 bp	1.0	53.1
rps3-rps19_p4	CTGAAACCAAGCGGATTTATT	TAAACTTCCGAGGACATGCG	38.1	50.0	54.8	58.0	541-557 bp	1.0	52.8
rps3-rps19_p5	CTGAAACCAAGCGGATTTAT	CCGAGGACATGCGAAAAATG	40.0	50.0	54.0	57.8	534-550 bp	1.0	52.6